gfp signal measurement  (Tecan Systems)

Journal: Biology

Article Title: Antitumor Effect of Cabozantinib in Bone Metastatic Models of Renal Cell Carcinoma

doi: 10.3390/biology10080781

Figure Lengend Snippet: Representative schemes of OBs/RCC tumor cell coculture models. Model ( A ): 786-O GFP+ cells and GFP+ Caki-1 cells were seeded on an OB layer and treated or not with cabozantinib at 5 μM. After 7 dd, the GFP signal of the RCC cells was measured. Model ( B ): The OBs were treated or not with cabozantinib at 5 μM for 7 dd. GFP+ RCC cells were added, and the GFP signal was measured after 7 dd.

Article Snippet: In both coculture models, the GFP signal of the RCC cells was measured after 2 h from the seeding (t0) and after 7 dd using using Nikon NIS-Elements microscope imaging software.

Techniques:

Journal: Biology

Article Title: Antitumor Effect of Cabozantinib in Bone Metastatic Models of Renal Cell Carcinoma

doi: 10.3390/biology10080781

Figure Lengend Snippet: Effects of OBs on metastatic RCC cell proliferation. ( A ) Representative images of Caki-1 GFP+ (green) and 786-O GFP+ cells (green) cultured alone or with OBs. Scale bar: 100 μM and magnification 4×. ( B ) Proliferation rate analysis of Caki-1 GFP+ and 786-O GFP+ cells in a monoculture or cocultured with OBs. Data are expressed as the mean ± SD; *** p < 0.001.

Article Snippet: In both coculture models, the GFP signal of the RCC cells was measured after 2 h from the seeding (t0) and after 7 dd using using Nikon NIS-Elements microscope imaging software.

Techniques: Cell Culture

Journal: Biology

Article Title: Antitumor Effect of Cabozantinib in Bone Metastatic Models of Renal Cell Carcinoma

doi: 10.3390/biology10080781

Figure Lengend Snippet: Antitumor effect of cabozantinib in RCC cells. Viability assay (MTT) of Caki-1 ( A ) and 786-O cells ( B ) after treatments with different doses of cabozantinib (10 nM, 100 nM, 500 nM, 1 μM and 5 μM). Data are expressed as the mean ± SD; * p ≤ 0.05.

Article Snippet: In both coculture models, the GFP signal of the RCC cells was measured after 2 h from the seeding (t0) and after 7 dd using using Nikon NIS-Elements microscope imaging software.

Techniques: Viability Assay

Journal: Biology

Article Title: Antitumor Effect of Cabozantinib in Bone Metastatic Models of Renal Cell Carcinoma

doi: 10.3390/biology10080781

Figure Lengend Snippet: Antitumor effect of cabozantinib in the RCC bone microenvironment. Proliferation ( A ) and inhibition rates ( B ) of Caki-1 GFP+ (left panels) and 786-O GFP+ (right panels) cells after a cabozantinib treatment (5 μM) in a monoculture or cocultured with OBs. Data are expressed as the mean ± SD; * p ≤ 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

Article Snippet: In both coculture models, the GFP signal of the RCC cells was measured after 2 h from the seeding (t0) and after 7 dd using using Nikon NIS-Elements microscope imaging software.

Techniques: Inhibition